Concern about the accumulation of chemicals in feed also involves additives used to prevent oxidation of fats included in animal feed. For this, the antioxidant effect of the cimenol ring, active principle from plant extracts, has been evaluated.

Mechanism of action

Cimenol ring has an antioxidant effect: it prevents fat oxidation and maintains feed nutritional and organoleptic properties. Its mechanism of action consists in:

1. Cimenol ring is more reactive than nutrients in peroxidation process, so that it is oxidized in their place in presence of free radicals.

2. Neutralizes electrons released during peroxidation and prevents degradation.

A- Oxidized feed, free and reactive radicals. A Feed without free electrons, it is not reactive: oxidation chain is disrupted. e- Electron

Comparative with Vitamin E

Vitamin E is a fat-soluble nutrient present in feed that acts as a natural antioxidant, so that it is used as a pattern to compare the antioxidant activity of other molecules.

Aim of the trial

To compare the antioxidant capacity of the cimenol ring with vitamin E (natural antioxidant).

Methodology

Spectrophotometry is conducted using DPPH as an indicator for oxidation. In a solution, DPPH is purple, but, when it interacts with an antioxidant, the unpaired electron is neutralized and the resulting molecules is colorless. Therefore, the lowest the absorbance measured in the spectrophotometer, the higher is the capacity to neutralize free radicals of the evaluated substance (higher antioxidant capacity).

Prepare the solutions to be tested:

• 8 ml with the following concentrations of vitamin E: 0.05 g/ml, 0.005 g/ml, 5×10-4 g/ml, 5×10-5 g/ml, 5×10-6 g/ml.
• 8 ml with the following concentrations of cimenol: 0.05 g/ml, 0.005 g/ml, 5×10-4 g/ml, 5×10-5 g/ml, 5×10-6 g/ml.

Design the calibration curve with DPPH (absorbance at 517 nm/DPPH concentration in mM)

Results

Results obtained at 0.05 g/ml and 0.005 g/ml can’t be compared because the 100% of DPPH is neutralized, which means there is too much antioxidant compound (cimenol) in the sample.

Conclusions

Cimenol ring is more effective than vitamin E as an antioxidant: at a concentration of 5×10^-4 g/ml (0.5 mg/l) is 41.52 times more effective than vitamin E at the same concentration.

Therefore, this compound can be used as an antioxidant in raw materials and balanced feed for feed at much lower doses than vitamin E.

Products with cimenol ring

Alquernat Oxyfree and Alquermold Natural, two products marketed by Biovet, S.A., contain cimenol ring at different concentrations. So, at a dose rate of 500 g of product per ton of feed:

• Alquernat Oxyfree is 41.52 times more effective than vitamin E
• Alquermold Natural is 12.76 times more effective than vitamin E