{"id":56800,"date":"2019-03-14T11:57:51","date_gmt":"2019-03-14T10:57:51","guid":{"rendered":"https:\/\/biovet-alquermes.com\/intestinal-conditioner-induced-identification-of-genes-and-tissue-specific-proteins\/"},"modified":"2024-04-22T12:13:44","modified_gmt":"2024-04-22T10:13:44","slug":"intestinal-conditioner-induced-identification-of-genes-and-tissue-specific-proteins","status":"publish","type":"post","link":"https:\/\/biovet-alquermes.com\/en\/2019\/03\/14\/intestinal-conditioner-induced-identification-of-genes-and-tissue-specific-proteins\/","title":{"rendered":"Identification of genes and tissue-specific proteins induced by an intestinal conditioner"},"content":{"rendered":"

\"Identification<\/p>\n

Effect of intestinal conditioner pronutrients on expression of genes related to intestinal mucosa status<\/strong> was evaluated on an in vitro<\/em> culture of enterocytes.<\/p>\n

Methodology<\/b><\/span><\/h2>\n

Procedure for obtaining specific tissue-proteins that have increased expression thanks to the use of intestinal conditioner pronutrients is based on the following stages:<\/p>\n

1) Development of two cellular cultures of porcine enterocytes: one culture is a control (without treatment) and another culture is treated with an intestinal conditioner.<\/p>\n

2) Isolation of total RNA from treated and untreated enterocytes.<\/p>\n

3) Construction of a complete transcriptomic library and RNA sequencing.<\/p>\n

4) Bioinformatic analysis: analysis of the differential expression between treated and untreated cultures, showed as volcano plot and heat map (Pictures 1 and 2<\/em>).<\/p>\n

5) Data review: search in databases about the biological function of differentially expressed genes, to determine molecular basis of the beneficial effect of an intestinal conditioner on enterocytes.<\/p>\n

\"Comparaci\u00f3n

Picture 1. Volcano plot – Comparison between gene expression of control and treated cultures.<\/span><\/p><\/div>\n

Volcano plot is a scatter diagram of -log10 (p) (on the vertical axis) as a function of M (on the horizontal axis). Genes that are expressed differently (statistically significant difference) and, therefore, with “fold change”, will tend to be found at the upper right and left sides of the chart.<\/p>\n

\"

Picture 2. Heat map – Comparison between gene expression of control and intestinal conditioner cultures.<\/span><\/p><\/div>\n

In the case of the heat map, samples are grouped into control samples (right) and treated samples (left), and difference in expression is shown through a color code.<\/p>\n

Results<\/span><\/strong><\/h2>\n
\"Genes

Picture 3. Genes induced by the presence of an intestinal conditioner. <\/span><\/p><\/div>\n

Treatment of porcine enterocytes in vitro with an intestinal conditioner increases the expression of certain genes related to:<\/strong><\/span><\/h3>\n